Western blot analysis of extracts of U-251MG cells, using PCOLCE antibody (A15298) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
PCOLCE Rabbit pAb
Catalog No:AB-A15298
Manufacturer:ABclonal
Synonyms:PCOLCE;PCPE;PCPE-1;PCPE1
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PCOLCE Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-449 of human PCOLCE (NP_002584.2). |
Gene ID: | 5118 |
Swiss prot: | Q15113 |
Molecular weight: | 47kDa |
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