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Western blot analysis of extracts of various cell lines, using PCNA antibody (A13336) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts of various cell lines, using PCNA antibody (A13336) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
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Immunohistochemistry of paraffin-embedded human liver cancer using PCNA antibody (A13336) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using PCNA antibody (A13336) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using PCNA antibody (A13336) at dilution of 1:100 (40x lens).
PCNA Rabbit pAb
Catalog No：AB-A13336
Manufacturer：ABclonal
Synonyms：PCNA;ATLD2
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | PCNA Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PCNA (NP_002583.1). |
| Gene ID: | 5111 |
| Swiss prot: | P12004 |
| Molecular weight: | 28kDa |
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