Western blot analysis of extracts of various cell lines, using PCK1 antibody (A2036) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of HepG2 cells, using PCK1 antibody (A2036) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
PCK1 Rabbit pAb
Catalog No:AB-A2036
Manufacturer:ABclonal
Synonyms:PCK1;PEPCK-C;PEPCK1;PEPCKC
Applications:WB,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PCK1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 520 to the C-terminus of human PCK1 (NP_002582.3). |
Gene ID: | 5105 |
Swiss prot: | P35558 |
Molecular weight: | 34kDa/69kDa |
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