Western blot analysis of extracts of various cell lines, using PCDHGC3 antibody (A10235) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 20s.
PCDHGC3 Rabbit pAb
Catalog No:AB-A10235
Manufacturer:ABclonal
Synonyms:PCDHGC3;PC43;PCDH-GAMMA-C3;PCDH2
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PCDHGC3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 32-270 of human PCDHGC3 (NP_002579.2). |
Gene ID: | 5098 |
Swiss prot: | Q9UN70 |
Molecular weight: | 94kDa/101kDa |
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