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Western blot analysis of extracts of various cell lines, using PCCA antibody (A9486) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
PCCA Rabbit pAb
Catalog No：AB-A9486
Manufacturer：ABclonal
Synonyms：PCCA
Applications：WB
Reactivity：Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | PCCA Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 449-728 of human PCCA (NP_000273.2). |
| Gene ID: | 5095 |
| Swiss prot: | P05165 |
| Molecular weight: | 75kDa/77kDa/80kDa |
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