Western blot analysis of extracts of various cell lines, using PAX6 antibody (A7334) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 30s.
PAX6 Rabbit pAb
Catalog No:AB-A7334
Manufacturer:ABclonal
Synonyms:AN;AN2;ASGD5;D11S812E;FVH1;MGDA;WAGR;PAX6
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PAX6 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter’s anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PAX6 (NP_000271.1). |
Gene ID: | 5080 |
Swiss prot: | P26367 |
Molecular weight: | 46kDa/48kDa |
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