Western blot analysis of extracts of various cell lines, using pan-Tri-Methyl K Motif antibody (A20145) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.

Immunoprecipitation analysis of 1.5mg extracts of HeLa cells using 30ug pan-Tri-Methyl lysine antibody (A20145). Western blot was performed from the immunoprecipitate using TriMethyl-Histone H3-K27 antibody (A2363) at a dilition of 1:1000.