Western blot analysis of extracts of Jurkat cells, using Pan Phospho-Tyrosine pAb (AP0905) at 1:1000 dilution.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.

Immunofluorescence analysis of C6 cells using Pan Phospho-Tyrosine (AP0905) at dilution of 1:100. Blue: DAPI for nuclear staining.C6 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.

Immunofluorescence analysis of NIH/3T3 cells using Pan Phospho-Tyrosine (AP0905) at dilution of 1:100. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by 20% FBS at 37℃ for 20 minutes after serum-starvation overnight.

Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Pan Phospho-Tyrosine antibody (AP0905). Western blot was performed from the immunoprecipitate using Pan Phospho-Tyrosine antibody (AP0905) at a dilition of 1:1000.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.