Western blot analysis of extracts of various cell lines, using pan-Mono-Methyl K Motif antibody (A20505) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.

Chromatin immunoprecipitation analysis of extracts of K-562 cells, using Pan Mono-Methyl lysine antibody (A20505) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Immunoprecipitation analysis of 1000ug extracts of HeLa cells using 20ug Pan Mono-Methyl lysine antibody (A20505). Western blot was performed from the immunoprecipitate using MonoMethyl-Histone H3-K9 (A2358) at a dilition of 1:1000.