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Western blot analysis of extracts of various cell lines, using p53 antibody (A5804) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
p53 Rabbit pAb
Catalog No：AB-A5804
Manufacturer：ABclonal
Synonyms：TP53;BCC7;LFS1;P53;TRP53;Trp53;bbl;bfy;bhy;p44;p53;Tp53
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | p53 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 95-390 of mouse p53 (NP_035770.2). |
| Gene ID: | 22059 |
| Swiss prot: | P02340 |
| Molecular weight: | 43kDa |
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