Western blot analysis of extracts of U-87MG cells, using P3H4 antibody (A13754) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
P3H4 Rabbit pAb
Catalog No:AB-A13754
Manufacturer:ABclonal
Synonyms:P3H4;LEPREL4;NO55;NOL55;SC65
Applications:WB
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | P3H4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This nucleolar protein was first characterized because it was an autoantigen in cases on interstitial cystitis. The protein, with a predicted molecular weight of 50 kDa, appears to be localized in the particulate compartment of the interphase nucleolus, with a distribution distinct from that of nucleolar protein B23. During mitosis it is associated with chromosomes. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 338-437 of human P3H4 (NP_006446.1). |
Gene ID: | 10609 |
Swiss prot: | Q92791 |
Molecular weight: | 50kDa |
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