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Western blot analysis of extracts of various cell lines, using Osteopontin antibody (A1361) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Osteopontin Rabbit pAb
Catalog No：AB-A1361
Manufacturer：ABclonal
Synonyms：BNSP;BSPI;ETA-1;OPN;Osteopontin;SPP1
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Osteopontin Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 17-314 of human Osteopontin (NP_001035147.1). |
| Gene ID: | 6696 |
| Swiss prot: | P10451 |
| Molecular weight: | 32kDa/33kDa/35kDa |
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