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Western blot analysis of extracts of various cell lines, using OGDH Antibody (A12477) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using OGDH antibody (A12477) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using OGDH antibody (A12477) at dilution of 1:100 (40x lens).
OGDH Rabbit pAb
Catalog No：AB-A12477
Manufacturer：ABclonal
Synonyms：OGDH;AKGDH;E1k;OGDC
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | OGDH Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human OGDH (NP_001003941.1). |
| Gene ID: | 4967 |
| Swiss prot: | Q02218 |
| Molecular weight: | 48kDa/115kDa |
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