Western blot analysis of extracts of various cell lines, using NXF1 Rabbit mAb (A3488) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
NXF1 Rabbit mAb
Catalog No:AB-A3488
Manufacturer:ABclonal
Synonyms:MEX67, TAP
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | NXF1 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human NXF1 |
Gene ID: | 10482 |
Swiss prot: | Q9UBU9 |
Molecular weight: | 70kDa |
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