Nt.BspQI
Catalog No：AB-RK21300
Manufacturer：ABclonal
Applications：DNA or RNA Ligases
€45,00 – €195,00
Description
3’…CGAGAAGN…5′
Product Components
| Components | Cat. No. |
|---|---|
| Nt.BspQI (10, 000 U/mL) | AB-RM21701 |
| 10X Buffer CutC | AB-RM20106 |
Product Information
| Featured | Specification | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Applications | Restriction Enzyme Digestion | ||||||||
| Unit Definition | One unit is defined as the amount of enzyme required to convert 1 μg of supercoiled pUC19 DNA to open circular form in 1 hour at 50°C in a total reaction volume of 50 µL. | ||||||||
| Storage Conditions | 10 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 μg/mL BSA, 50% Glycerol, pH 7.4 @ 25°C | ||||||||
| Storage Temperature | -20°C | ||||||||
| Reaction Conditions | 1X Buffer CutC. Incubate at 50°C. | ||||||||
| Reaction Buffer or Mix | 1X Buffer CutC: 100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 100 μg/mL BSA, pH 7.9 @ 25°C |
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| Heat Inactivation | 80°C for 20 min | ||||||||
| Methylation Sensitivity |
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| Activity in Abclonal Buffers |
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