Western blot analysis of extracts of various cell lines, using NSD1 antibody (A9981) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
NSD1 Rabbit pAb
Catalog No:AB-A9981
Manufacturer:ABclonal
Synonyms:ARA267;KMT3B;SOTOS;SOTOS1;STO;NSD1
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | NSD1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein containing a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals (NLSs), 4 plant homeodomain (PHD) finger regions, and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation, and this enhancement can be increased further in the presence of other androgen receptor associated coregulators. This protein may act as a nucleus-localized, basic transcriptional factor and also as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su-var, enhancer of zeste, and trithorax domain protein 1 on chromosome 5 and nucleoporin, 98-kd on chromosome 11. Two transcript variants encoding distinct isoforms have been identified for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-260 of human NSD1 (NP_071900.2). |
Gene ID: | 64324 |
Swiss prot: | Q96L73 |
Molecular weight: | 267kDa/284kDa/296kDa |
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