.jpg)
Western blot analysis of extracts of mouse kidney, using NPHS1 antibody (A3048) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
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Western blot analysis of extracts of various cell lines, using NPHS1 antibody (A3048) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded mouse kidney using NPHS1 antibody (A3048) (40x lens).
NPHS1 Rabbit pAb
Catalog No：AB-A3048
Manufacturer：ABclonal
Synonyms：NPHS1;CNF;NPHN;nephrin
Applications：WB,IHC,IF
Reactivity：Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | NPHS1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes. |
| Immunogen: | A synthetic peptide of human NPHS1 |
| Gene ID: | 4868 |
| Swiss prot: | O60500 |
| Molecular weight: | 130kDa/134kDa |
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