Western blot analysis of extracts of various cell lines, using NOX2/gp91phox antibody (A12430) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
NOX2/gp91phox Rabbit pAb
Catalog No:AB-A12430
Manufacturer:ABclonal
Synonyms:AMCBX2;CGD;GP91-1;GP91-PHOX;GP91PHOX;IMD34;NOX2;p91-PHOX;CYBB
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | NOX2/gp91phox Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell’s respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human NOX2/gp91phox (NP_000388.2). |
Gene ID: | 1536 |
Swiss prot: | P04839 |
Molecular weight: | 65kDa |
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