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Western blot analysis of extracts of various cell lines, using Notch1 antibody (A19090) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
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Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug Notch1 antibody (A19090). Western blot was performed from the immunoprecipitate using Notch1 antibody (A19090) at a dilition of 1:1000.
Notch1 Rabbit mAb
Catalog No：AB-A19090
Manufacturer：ABclonal
Synonyms：AOS5;AOVD1;TAN1;hN1;NOTCH1;Activated NOTCH1;notch 1
Applications：WB,IP
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | Notch1 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma. [provided by RefSeq, Jan 2016] |
| Immunogen: | A synthesized peptide derived from human Notch1 |
| Gene ID: | 4851 |
| Swiss prot: | P46531 |
| Molecular weight: | 125kDa |
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