Western blot analysis of extracts of various cell lines, using NGLY1 antibody (A15864) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
NGLY1 Rabbit pAb
Catalog No:AB-A15864
Manufacturer:ABclonal
Synonyms:NGLY1;CDDG;CDG1V;PNG1;PNGase
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | NGLY1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NGLY1 (NP_060767.2). |
Gene ID: | 55768 |
Swiss prot: | Q96IV0 |
Molecular weight: | 14kDa/63kDa/70kDa/72kDa/74kDa |
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