Western blot analysis of extracts of various cell lines, using NFYB antibody (A7295) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
NFYB Rabbit pAb
Catalog No:AB-A7295
Manufacturer:ABclonal
Synonyms:NFYB;CBF-A;CBF-B;HAP3;NF-YB
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | NFYB Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human NFYB (NP_006157.1). |
Gene ID: | 4801 |
Swiss prot: | P25208 |
Molecular weight: | 22kDa |
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