Western blot analysis of extracts of various cell lines, using NELFE antibody (A3249) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
NELFE Rabbit pAb
Catalog No:AB-A3249
Manufacturer:ABclonal
Synonyms:NELFE;D6S45;NELF-E;RD;RDBP;RDP
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | NELFE Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NELFE (NP_002895.3). |
Gene ID: | 7936 |
Swiss prot: | P18615 |
Molecular weight: | 40kDa/43kDa |
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