Western blot analysis of extracts of various cell lines, using NEDD4 antibody (A0552) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 30s.
NEDD4 Rabbit pAb
Catalog No:AB-A0552
Manufacturer:ABclonal
Synonyms:NEDD4;NEDD4-1;RPF1
Applications:WB,IHC
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | NEDD4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-460 of human NEDD4 (NP_940682.2). |
Gene ID: | 4734 |
Swiss prot: | P46934 |
Molecular weight: | 104kDa/140kDa/147kDa/149kDa |
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