Western blot analysis of extracts of various cell lines, using NAGS antibody (A12086) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
NAGS Rabbit pAb
Catalog No:AB-A12086
Manufacturer:ABclonal
Synonyms:NAGS;AGAS;ARGA
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | NAGS Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The N-acetylglutamate synthase gene encodes a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAG is a cofactor of carbamyl phosphate synthetase I (CPSI), the first enzyme of the urea cycle in mammals. This gene may regulate ureagenesis by altering NAG availability and, thereby, CPSI activity. Deficiencies in N-acetylglutamate synthase have been associated with hyperammonemia. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 295-534 of human NAGS (NP_694551.1). |
Gene ID: | 162417 |
Swiss prot: | Q8N159 |
Molecular weight: | 58kDa |
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