Western blot analysis of extracts of various cell lines, using MRPL12 antibody (A8318) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
MRPL12 Rabbit pAb
Catalog No:AB-A8318
Manufacturer:ABclonal
Synonyms:MRPL12;5c5-2;L12mt;MRP-L31/34;MRPL7;MRPL7/L12;RPML12
Applications:WB,IHC
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MRPL12 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human MRPL12 (NP_002940.2). |
Gene ID: | 6182 |
Swiss prot: | P52815 |
Molecular weight: | 21kDa |
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