Western blot analysis of extracts of K-562 cells, using MRE11 antibody (A2559) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Western blot analysis of extracts of various cell lines, using MRE11 antibody (A2559) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
MRE11 Rabbit pAb
Catalog No:AB-A2559
Manufacturer:ABclonal
Synonyms:MRE11;ATLD;HNGS1;MRE11A;MRE11B
Applications:WB,IHC,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MRE11 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3′ to 5′ exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3′ to 5′ exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human MRE11 (NP_005581.2). |
Gene ID: | 4361 |
Swiss prot: | P49959 |
Molecular weight: | 77kDa/80kDa/81kDa |
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