Western blot analysis of extracts of HL-60 cells, using MPO antibody (A1374) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
MPO Rabbit pAb
Catalog No:AB-A1374
Manufacturer:ABclonal
Synonyms:MPO
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MPO Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 50-310 of human MPO (NP_000241.1). |
Gene ID: | 4353 |
Swiss prot: | P05164 |
Molecular weight: | 73kDa/83kDa/87kDa |
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