Western blot analysis of extracts of over-expressed HA-tagged protein in 293T cells, using Mouse anti HA-Tag mAb (AE065) at 1:100000 dilution.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Mouse anti HA-Tag mAb
Catalog No:AB-AE065
Manufacturer:ABclonal
Synonyms:HA;HA tag;HA-tag
Applications:WB,IP
Host:Mouse
Conjugate:No
€70,00 – €185,00
Product Name: | Mouse anti HA-Tag mAb |
Category: | Tag and Loading Control Antibodies |
Description: | Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification. |
Immunogen: | A synthetic peptide corresponding to HA tag. |
Gene ID: | |
Swiss prot: | |
Molecular weight: |
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