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Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-R2 antibody (A19645) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-R2 antibody (A19645) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
MonoMethyl-Histone H3-R2 Rabbit mAb
Catalog No：AB-A19645
Manufacturer：ABclonal
Synonyms：H3/A;H3C2;H3C3;H3C4;H3C6;H3C7;H3C8;H3FA;H3C10;H3C11;H3C12;HIST1H3A
Applications：WB,ChIP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | MonoMethyl-Histone H3-R2 Rabbit mAb |
| Category: | Methylated Antibodies |
| Description: | This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. |
| Immunogen: | A synthesized peptide derived from human Histone H3 (mono methyl R2). |
| Gene ID: | 8290/8350 |
| Swiss prot: | Q16695/P68431 |
| Molecular weight: | 15kDa |
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