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Western blot analysis of extracts of various cell lines, using MonoMethyl-H2B-E105 antibody (A20230) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
MonoMethyl-H2B-E105 Rabbit pAb
Catalog No：AB-A20230
Manufacturer：ABclonal
Synonyms：H2B.1; H2B/f; H2BFF; HIST1H2BB
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | MonoMethyl-H2B-E105 Rabbit pAb |
| Category: | Methylated Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. |
| Immunogen: | A synthetic monomethylated peptide around E105 of human H2B. |
| Gene ID: | 3018 |
| Swiss prot: | P33778 |
| Molecular weight: | 14kDa |
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