Western blot analysis of extracts of various cell lines, using MLH3 antibody (A7796) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
MLH3 Rabbit pAb
Catalog No:AB-A7796
Manufacturer:ABclonal
Synonyms:MLH3;HNPCC7
Applications:WB,IF
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MLH3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1180-1429 of human MLH3 (NP_055196.2). |
Gene ID: | 27030 |
Swiss prot: | Q9UHC1 |
Molecular weight: | 161kDa/163kDa |
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