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Western blot analysis of extracts of HeLa cells, using MID1 Rabbit pAb (A7291) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
MID1 Rabbit pAb
Catalog No：AB-A7291
Manufacturer：ABclonal
Synonyms：MID1;BBBG1;FXY;GBBB1;MIDIN;OGS1;OS;OSX;RNF59;TRIM18;XPRF;ZNFXY;midline 1
Applications：WB,IF
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | MID1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the ‘RING-B box-coiled coil’ (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Alternative promoter use, alternative splicing and alternative polyadenylation result in multiple transcript variants that have different tissue specificities. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 478-667 of human MID1 (NP_000372.1). |
| Gene ID: | 4281 |
| Swiss prot: | O15344 |
| Molecular weight: | 62kDa/75kDa |
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