Western blot analysis of extracts of various cell lines, using MGMT Rabbit mAb (A11151) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
MGMT Rabbit mAb
Catalog No:AB-A11151
Manufacturer:ABclonal
Synonyms:MGMT; O-6-methylguanine-DNA methyltransferase
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | MGMT Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015] |
Immunogen: | A synthesized peptide derived from human MGMT |
Gene ID: | 4255 |
Swiss prot: | P16455 |
Molecular weight: | 22kDa |
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