Western blot analysis of extracts of various cell lines, using MGEA5 antibody (A9033) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
MGEA5 Rabbit pAb
Catalog No:AB-A9033
Manufacturer:ABclonal
Synonyms:MGEA5;MEA5;NCOAT;OGA
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MGEA5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 380-580 of human MGEA5 (NP_001135906.1). |
Gene ID: | 10724 |
Swiss prot: | O60502 |
Molecular weight: | 76kDa/95kDa/96kDa/102kDa |
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