Western blot analysis of extracts of various cell lines, using MEKK3 antibody (A16058) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
MEKK3 Rabbit pAb
Catalog No:AB-A16058
Manufacturer:ABclonal
Synonyms:MAP3K3;MAPKKK3;MEKK3
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MEKK3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human MEKK3 (NP_976226.1). |
Gene ID: | 4215 |
Swiss prot: | Q99759 |
Molecular weight: | 70kDa/74kDa |
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