Western blot analysis of extracts of various cell lines, using MEK5 antibody (A6953) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 20s.
MEK5 Rabbit pAb
Catalog No:AB-A6953
Manufacturer:ABclonal
Synonyms:MAP2K5;HsT17454;MAPKK5;MEK5;PRKMK5
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MEK5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MEK5 (NP_660143.1). |
Gene ID: | 5607 |
Swiss prot: | Q13163 |
Molecular weight: | 46kDa/48kDa/49kDa/50kDa |
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