Western blot analysis of extracts of various cell lines, using MED25 antibody (A16150) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
MED25 Rabbit pAb
Catalog No:AB-A16150
Manufacturer:ABclonal
Synonyms:MED25;ACID1;ARC92;BVSYS;CMT2B2;P78;PTOV2;TCBAP0758
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MED25 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human MED25 (NP_112235.2). |
Gene ID: | 81857 |
Swiss prot: | Q71SY5 |
Molecular weight: | 55kDa/76kDa/78kDa/82kDa/84kDa |
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