Western blot analysis of extracts of various cell lines, using MED20 antibody (A15757) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
MED20 Rabbit pAb
Catalog No:AB-A15757
Manufacturer:ABclonal
Synonyms:MED20;PRO0213;TRFP;SRB2
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MED20 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human MED20 (NP_004266.2). |
Gene ID: | 9477 |
Swiss prot: | Q9H944 |
Molecular weight: | 13kDa/23kDa |
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