Western blot analysis of extracts of various cell lines, using MCM5 antibody (A5556) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
MCM5 Rabbit pAb
Catalog No:AB-A5556
Manufacturer:ABclonal
Synonyms:MCM5;CDC46;P1-CDC46
Applications:WB,IF,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MCM5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2). |
Gene ID: | 4174 |
Swiss prot: | P33992 |
Molecular weight: | 82kDa |
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