Western blot analysis of extracts of various cell lines, using MARCH9 antibody (A10596) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
MARCH9 Rabbit pAb
Catalog No:AB-A10596
Manufacturer:ABclonal
Synonyms:MARCH9;MARCH-IX;RNF179
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MARCH9 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | MARCH9 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH9 induces internalization of several membrane glycoproteins and directs them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Hoer et al., 2007 [PubMed 17174307]).[supplied by OMIM, Apr 2010] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 277-346 of human MARCH9 (NP_612405.2). |
Gene ID: | 92979 |
Swiss prot: | Q86YJ5 |
Molecular weight: | 25kDa/37kDa |
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