Western blot analysis of extracts of various cell lines, using MARCH8 antibody (A12962) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
MARCH8 Rabbit pAb
Catalog No:AB-A12962
Manufacturer:ABclonal
Synonyms:MARCH8;CMIR;MARCH-VIII;MIR;RNF178;c-MIR
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MARCH8 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]). |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 232-291 of human MARCH8 (NP_659458.2). |
Gene ID: | 220972 |
Swiss prot: | Q5T0T0 |
Molecular weight: | 32kDa/63kDa |
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