Western blot analysis of extracts of 293T cells, using MAP3K8 antibody (A15623) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
MAP3K8 Rabbit pAb
Catalog No:AB-A15623
Manufacturer:ABclonal
Synonyms:MAP3K8;AURA2;COT;EST;ESTF;MEKK8;TPL2;Tpl-2;c-COT
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MAP3K8 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP3K8 (NP_005195.2). |
Gene ID: | 1326 |
Swiss prot: | P41279 |
Molecular weight: | 49kDa/52kDa |
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