Western blot analysis of extracts of various cell lines, using MAP3K12 antibody (A8464) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
MAP3K12 Rabbit pAb
Catalog No:AB-A8464
Manufacturer:ABclonal
Synonyms:MAP3K12;DLK;MEKK12;MUK;ZPK;ZPKP1
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MAP3K12 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human MAP3K12 (NP_001180440.1). |
Gene ID: | 7786 |
Swiss prot: | Q12852 |
Molecular weight: | 93kDa/96kDa |
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