Western blot analysis of extracts of various cell lines, using LysRS/LysRS/KARS antibody (A13772) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
LysRS/KARS Rabbit pAb
Catalog No:AB-A13772
Manufacturer:ABclonal
Synonyms:KARS;CMTRIB;DFNB89;KARS1;KARS2;KRS
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | LysRS/KARS Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 298-597 of human LysRS/LysRS/KARS (NP_005539.1). |
Gene ID: | 3735 |
Swiss prot: | Q15046 |
Molecular weight: | 68kDa/71kDa |
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