Western blot analysis of extracts of various cell lines, using Leptin Receptor antibody (A2999) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Leptin Receptor Rabbit pAb
Catalog No:AB-A2999
Manufacturer:ABclonal
Synonyms:CD295;LEP-R;LEPRD;OB-R;OBR;LEPR
Applications:WB,IF
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Leptin Receptor Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021). |
Immunogen: | A synthetic peptide of human LEPR |
Gene ID: | 3953 |
Swiss prot: | P48357 |
Molecular weight: | 96kDa/102kDa/103kDa/109kDa/132kDa |
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