Western blot analysis of extracts of various cell lines, using Legumain (Legumain (LGMN)) antibody (A10570) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Legumain (LGMN) Rabbit pAb
Catalog No:AB-A10570
Manufacturer:ABclonal
Synonyms:LGMN;AEP;LGMN1;PRSC1;legumain
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Legumain (LGMN) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-323 of human Legumain (Legumain (LGMN)) (NP_001008530.1). |
Gene ID: | 5641 |
Swiss prot: | Q99538 |
Molecular weight: | 42kDa/49kDa |
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