Western blot analysis of extracts of various cell lines, using LDHB antibody (A7625) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
LDHB Rabbit pAb
Catalog No:AB-A7625
Manufacturer:ABclonal
Synonyms:LDHB;HEL-S-281;LDH-B;LDH-H;LDHBD;TRG-5
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | LDHB Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human LDHB (NP_002291.1). |
Gene ID: | 3945 |
Swiss prot: | P07195 |
Molecular weight: | 36kDa |
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