Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (A15591) at 1:1000 dilution. Hela cells were treated by Chloroquine (50 μM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 μM) for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
LC3A / LC3B Rabbit pAb
Catalog No:AB-A15591
Manufacturer:ABclonal
Synonyms:MAP1LC3B;ATG8F;LC3B;MAP1A/1BLC3;MAP1LC3B-a;MAP1LC3A;ATG8E;LC3;LC3A;MAP1ALC3;MAP1BLC3;LC3A/LC3B
Applications:WB,IHC,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | LC3A / LC3B Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human LC3A / LC3B (NP_073729.1). |
Gene ID: | 84557/81631 |
Swiss prot: | Q9GZQ8/Q9H492 |
Molecular weight: | 14kDa |
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