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Western blot analysis of extracts of C6 cells, using Lactic acid-Histone H3-K27 antibody (A18825) at 1:1000 dilution.C6 cells were treated by Sodium lactate(100mM) for 24h.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Lactic acid-Histone H3-K27 Rabbit pAb
Catalog No：AB-A18825
Manufacturer：ABclonal
Synonyms：HIST1H3A; H3/A; H3FA; histone H3.1
Applications：WB
Reactivity：Human,Mouse,Rat,Other (Wide Range)
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Lactic acid-Histone H3-K27 Rabbit pAb |
| Category: | Other Modified Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. |
| Immunogen: | A synthetic peptide of human Lactic acid-Histone H3-K27. |
| Gene ID: | 8350 |
| Swiss prot: | P68431 |
| Molecular weight: | 15kDa |
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