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Western blot analysis of extracts of HepG2 cells, using YB-1/YB-1/YBX1 antibody (A7704) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Western blot analysis of extracts from normal (control) and YB-1/YB-1/YBX1 knockout (KO) HeLa cells, using YB-1/YB-1/YBX1 antibody (A7704) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
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Immunohistochemistry of paraffin-embedded rat testis using YB-1/YB-1/YBX1 antibody (A7704) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using YB-1/YB-1/YBX1 antibody (A7704) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using YB-1/YB-1/YBX1 antibody (A7704) at dilution of 1:100 (40x lens).
[KO Validated] YB-1/YBX1 Rabbit pAb
Catalog No：AB-A7704
Manufacturer：ABclonal
Synonyms：YBX1;BP-8;CBF-A;CSDA2;CSDB;DBPB;EFI-A;MDR-NF1;NSEP-1;NSEP1;YB-1;YB1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] YB-1/YBX1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YB-1/YB-1/YBX1 (NP_004550.2). |
| Gene ID: | 4904 |
| Swiss prot: | P67809 |
| Molecular weight: | 35kDa |
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